DAWN HELEOS II MALS (Multi Angle Light Scattering)
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Location - Britannia 107
For enquiries, please contact - Dr. Karin Smorodinsky-Atias
The information provided in this section is taken from the “Hardware Manual for the DAWN HELEOS Light Scattering Instrument” and "Optilab rEX User’s Guide" by Waters Corporation (Wyatt Technology).
Multi-angle static light scattering (MALS)
This method is used to determine the molar mass, conformation and interactions of molecules in solution.
A sample is illuminated using a laser beam as a light souse. The light is scattered by the sample at all angles, 18 of which are simultaneously detected by the MALS instrument.
The measured light scattering signals are integrated and analyzed by the software, yielding the absolute molar mass.
Application
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Determining absolute molecular weight
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Determining oligomeric state
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Determining stoichiometry
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Determining branching properties
Requirements
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The exact matched buffer/solvent of the measured sample
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All liquids should be filtered using a ≤0.02 µm filter
Advantages
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Doesn't relay on any standards
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Directly referenced to the sample solvent
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High sensitivity
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High dynamic range
