Prof. Michael Gurevitz

ביולוגיה מול.ואקול.צמחים אמריטוס
Prof. Michael Gurevitz
Phone: 03-6409844
External phone: 09-7717160
Fax: 03-6406100
Office: Britannia-Porter, 505

Research Interests

Molecular mechanisms underlying the mode of action of scorpion toxins and their
specificity for neuronal sodium channel subtypes

Regulation of cyanobacterial Ribulose 1,5-Bisphosphate Carboxylase/Oxygenase
(RuBisCO) by redox and inorganic phosphate

Recent Publications

Zhang, J.Z, Yarov-Yarovoy, V., Scheuer, T., Karbat, I., Cohen, L., Gordon, D., Gurevitz, M. and Catterall, W.A. (2012) Mapping the interaction site for a b -scorpion toxin in the pore module of domain III of voltage-gated sodium channels. J. Biol. Chem . , Epub ahead of print, PMID: 22761417.

 

Gur, M, Reitzel, A.M., Eitel, M., Schierwater, B., Genikhovich, G., Technau, U., Gordon, D., Gurevitz, M. and Moran, Y. (2012) Convergent evolution of sodium selectivity in metazoan voltage-gated ion channels. Cell Rep ., http://dx.doi.org/10.1016/j.celrep.2012.06.016.

 

Gurevitz, M. (2012) Mapping the scorpion toxin receptor sites at voltage-gated sodium channels. Toxicon , 60:502-511 .

 

Moran, Y., Genikhovich, G., Gordon, D., Technau, U. and Gurevitz, M. (2012) Localization of neurotoxins to ectodermal gland cells uncovers an alternative mechanism of venom delivery in sea anemones. Proc. Royal Soc. B ., 279: 1351 - 1358 .

 

Gur, M., Kahn, R., Karbat, I., Regev, N., Wang, J., Catterall, W.A., Gordon, D. and Gurevitz, M. (2011) Elucidation of the molecular basis of selective recognition uncovers the interaction site for the core-domain of scorpion alpha-toxins on sodium channels. J. Biol. Chem. , 286:35209-35217 .

 

Wang, J., Yarov-Yarovoy, V., Kahn, R., Gordon, D., Gurevitz, M., Scheuer, T. and Catterall, W.A. (2011) Molecular determinants for a -scorpion toxin binding to the resting state of a voltage sensor of brain sodium channels. Proc. Natl. Acad. Sci. USA , 108:15426-15431.

 

Zhang, Z., Karbat, I., Cohen, L., Yarov-Yarovoy, V., S cheuer, T., Gordon, D., Gurevitz, M. Catterall, W.A. (2011) Refined mapping of scorpion beta-toxin docking at domain 2 of a voltage-gated sodium channel. J. Biol. Chem. , 286:33641-33651.

 

Marcus, Y., Altman-Gueta, H., Wolff, Y. and Gurevitz, M. (2011) Rubisco mutagenesis provides new insight into limitations on photosynthesis and growth in SynechocystisPCC6803. J. Exp. Bot ., 62: 4173-4182 .

 

Song, W., Du, Y., Liu, Z., Luo, N., Turkov, M., Gordon, D., Gurevitz, M., Goldin, A.L. and Dong, K. (2011) Substitutions in the Domain III voltage sensor module III enhance the sensitivity of an insect sodium channel to a scorpion b -toxin. J. Biol. Chem. , 286:15781-15788.

 

Karbat, I., Ilan, N., Zhang, J.Z., Cohen, L., Kahn, R., Benveniste, M., Scheuer, T., Catterall, W.A., Gordon, D. and Gurevitz, M. (2010) Partial agonist and antagonist activities of a mutant scorpion b -toxin on sodium channels. J. Biol. Chem. , 285:30531-30538.

 

Paldi, T. and Gurevitz, M (2010) Coupling between residues on S4 and S1 define the voltage sensor resting conformation in NaChBac . Biophys. J. , 99:456-463.
Weinberger, H., Moran, Y., Kahn, R., Gordon, D. and Gurevitz, M. (2010) Positions under positive selection - key for selectivity and potency of scorpion a -toxins .Mol. Biol. Evol. , 27:1025-1034 .

 

Moran, Y., Gordon, D. and Gurevitz, M. (2009) Sea anemone toxins affecting voltage-gated sodium channels - molecular and evolutionary features . Toxicon , 54: 1089-1101 .

 

Cohen, L., Moran, Y., Sharon, A., Segal, D., Gordon, D. and Gurevitz, M. (2009) Drosomycin, an innate immunity peptide of Drosophila , interacts with DmNa v 1 of the nervous system. J. Biol. Chem. , 284:23558 - 235 63 .

 

Moran, Y., Weinberger, H., Lazarus, N., Gur, M., Kahn, R., Gordon, D. and Gurevitz, M. (2009) Fusion and retrotransposition events in the evolution of sea anemone neurotoxin genes. J. Mol. Evol. , 69:115-124.

 

Kahn, R., Karbat, I., Ilan, N., Cohen, L., Gordon, D. and Gurevitz, M. (2009) Molecular requirements for specific recognition of brain voltage-gated sodium channels by scorpion alpha-toxins. J. Biol. Chem. , 284:20684-20691 .

 

Moran, Y., Weinberger, H., Reitzel, A.M., Sullivan, J.C., Kahn, R., Gordon, D., Finnerty, J.R. and Gurevitz, M. (2008) Intron retention as a post-transcriptional regulatory mechanism of neurotoxin expression at early life stages of the starlet anemone Nematostella vectensis .J. Mol. Biol. , 380:437-443.

 

Marcus, Y., Altman-Gueta, H., Snir, A., Wolff, Y. and Gurevitz, M. (2008) Does Rubiscolimit the rate of photosynthesis? in 'Photosynthesis, Energy From The Sun (Allen, J.F., Gantt, E., Golbeck, J.H. and Osmond, B., eds.) 14 th International Congress on Photosynthesis, pp. 867-871, Springer Verlag.

 

Cohen, L., Lipstein, N., Karbat, I., Ilan, N., Gilles, N., Kahn, R., Gordon, D. and Gurevitz, M. (2008) Miniaturization of scorpion beta-toxins uncovers a putative ancestral surface of interaction with voltage-gated Na-channels. J. Biol. Chem. , 283:15169-15176 (paper of the week).

 

Moran, Y., Weinberger, H., Sullivan, J.C., Reitzel, A.M., Finnerty, J.R. and Gurevitz, M. (2008) 'Concerted Evolution' of sea anemone neurotoxin genes is revealed through analysis of the Nematostella vectensis genome. Mol. Biol. Evol. , 25 : 737-747 .

 

Goldberg, E., Turkov, M., Kahn, R., Gordon, D., Gurevitz, M. and Anglister , J. (2008) NMR analysis Lqh-alpha-IT scorpion toxin interaction with a peptide corresponding to the D4/S3-S4 loop of insect Para voltage-gated sodium channel . Biochem istry , 47:911-921 .

 

Cohen, L., Ilan, N., Gur, M., Stühmer, W., Gordon, D. and Gurevitz, M. (2007) Design of a specific activator for skeletal muscle voltage-gated sodium channels uncovers channel architecture. J. Biol. Chem. , 282:29424-29430 .

 

Cohen, L., Troub, Y., Turkov, M., Gilles, N., Ilan, N., Benveniste, M., Gordon, D. and Gurevitz, M. (2007) Mammalian skeletal muscle voltage-gated sodium channels are affected by scorpion depressant 'insect-selective' toxins when preconditioned. Mol. Pharmacol. , 72:1220-1227.

 

Moran, Y., Kahn, R., Cohen, L., Gur, M., Karbat, I., Gordon, D. and Gurevitz, M. (2007) Molecular analysis of the sea anemone toxin Av3 reveals selectivity to insects and demonstrates the heterogeneity of receptor site-3 on voltage-gated Na-channels . Biochem. J. , 406:41-48 .

 

Karbat, I., Kahn, R., Cohen, L., Ilan, N., Gilles, N., Corzo, G., Froy, O., Gur, M., Albrecht, G., Heinemann, S.H., Gordon, D. and Gurevitz, M. (2007) The unique pharmacology of the scorpion alpha-like toxin Lqh3 is associated with its flexible C-tail . FEBS J. , 274:1918-1231.

 

Gordon, D., Karbat, I., Ilan, N., Cohen, L., Kahn, R., Gilles, N., Dong, K., Stuehmer, W., Tytgat, J. and Gurevitz, M. (2007 ) The differential preference of scorpion alpha-toxins for insect or mammalian sodium channels: implications for improved insect control. Toxicon , 49:452-472.

 

Gurevitz, M., Karbat, I., Cohen, L., Ilan, N., Kahn, R., Turkov, M., Stankiewicz, M., Stuehmer, W., Dong, K, and Gordon, D. (2007) The insecticidal potential of scorpion beta-toxins. Toxicon , 49:473-489.

 

Karbat, I., Turkov, M., Cohen, L., Kahn, R., Gordon, D., Gurevitz, M. and Frolow, F. (2007) X-ray structure and mutagenesis of the scorpion depressant toxin LqhIT2 reveals key determinants crucial for activity and anti-insect selectivity . J. Mol. Biol. ­ , 366:586-601.

 

Snir, A., Gurevitz, M. and Marcus, Y. (2006 ) Photosynthetic diurnal rhythm in aerial leaves of the amphibious-plant Nuphar lutea is induced by alterations in Rubisco activity and stomatal behavior. Photosynthesis Res. , 90:233-242.

 

Regev, A. , Rivkin, H., Gurevitz, M, and Chejanovsky, N. (2006) New measures of insecticidal efficacy and safety obtained with the 39K promoter of a recombinant baculovirus. FEBS Lett . , 580:6777-6782.

 

Moran, Y. and Gurevitz, M. (2006) When positive selection of neurotoxin genes is missing - the riddle of the sea anemone Nematostella vectensis . FEBS J . , 276:3886-3892.

 

Moran, Y., Kahn, R., Cohen, L., Karbat, I., Ilan, N., Gordon, D. and Gurevitz, M. (2006)Expression and mutagenesis of the sea anemone toxin Av2 reveals key amino acid residues important for activity on insect voltage-gated sodium channels. Biochemistry , 45:8864-8873.

 

Cohen, L., Gilles, N., Karbat, I., Ilan, N., Gordon, D. and Gurevitz, M. (2006) Direct evidence that receptor site-4 of sodium channel gating modifiers is not dipped in the phospholipid bilayer of neuronal membranes. J. Biol. Chem. , 281:20673-20679.

 

Strugatsky, D., Zilberberg, N., Ilan, N., Turkov, M., Cohen, L., Stankiewicz, M., Pelhate, M., Gilles, N., Gordon, D. and Gurevitz, M. (2005) Expression of a gene family encoding a novel scorpion depressant toxin illuminates the key role of asparagine- 58 in activity on insect neuronal sodium channels. Biochemistry , 44:9179-9187.

 

Marcus, Y., Altman-Gueta, H., Finkler, A. and Gurevitz, M. (2005) Mutagenesis at two distinct phosphate-binding sites unravels their differential role in regulation of Rubisco activation and catalysis. J. Bacteriol. , 187:4222-4228.

 

Cohen, L., Karbat, I., Gilles, N., Ilan, N., Gordon, D. and Gurevitz, M. (2005) Common features in the functional surface of scorpion beta-toxins and elements that confer specificity for insect and mammalian voltage-gated Na-channels. J. Biol. Chem. , 280:5045-5053.

 

Corzo, G., Escoubas, E., Villegas, E., Karbat, I., Gordon, D., Gurevitz, M., Nakajima, T. and Gilles, N. (2005) A spider toxin that induces a typical effect of scorpion alpha-toxins but competes with beta-toxins on binding to insect sodium channels. Biochemistry , 44:1542-1549.

 

Tan, J., Liu, Z., Wang, R., Huang, Z., Chen, A. C., Gurevitz, M. and Dong, K. (2005) Identification of amino acid residues critical for pyrethroid binding to insect sodium channels. Mol. Pharmacol. , 67:513-522.

 

Karbat, I., Frolow, F., Froy, O., Gilles, N., Cohen, L., Turkov, M., Gordon, D. and Gurevitz, M. (2004) Molecular basis of the high insecticidal potency of scorpion alpha-toxins. J. Biol. Chem. , 279:31679-31686.

 

Froy, O. and Gurevitz, M. (2004) Arthropod defensins illuminate the divergence of scorpion neurotoxins. J. Pept. Sci. , 10:714-718.

 

Karbat, I., Cohen, L., Gilles, N., Gordon, D. and Gurevitz, M. (2004) Conversion of a scorpion toxin agonist into an antagonist highlights an acidic residue involved in voltage sensor trapping during activation of neuronal Na + channels. FASEB J. , 18:683-689.

 

Cohen, L., Karbat, I., Gilles, N., Froy, O., Corzo, G., Angelovici, R., Gordon, D. and Gurevitz, M. (2004) Dissection of the functional surface of an anti-insect excitatory toxin illuminates a putative ‘hot spot’ common to all scorpion beta-toxins affecting Na + channels. J. Biol. Chem. , 279:8206-8211.

 

Froy, O. and Gurevitz, M. (2003) Arthropod and Mollusk defensins - evolution by exon shuffling. Trends Genet . , 19:684-687.

 

Froy, O. and Gurevitz, M. (2003) New insight on scorpion divergence inferred from comparative analysis of toxin structure, pharmacology and distribution. Toxicon , 42:549-555.

 

Gordon, D., Zilberberg, N., Ilan, N., Gilles, N., Urbach, D., Cohen, L., Karbat, I., Froy, O., Gaathon, A., Kallen, R. G., Benveniste, M. and Gurevitz, M. (2003) An ‘Old World’ scorpion beta-toxin that recognizes both insect and mammalian sodium channels: a possible link towards diversification of beta-toxins. Eur. J. Biochem . , 270:2663-2670.

 

Gilles, N., Gurevitz, M. and Gordon, D. (2003) Allosteric interactions among pyrethroid, brevetoxin, and scorpion toxin receptors on insect sodium channels raise an alternative approach for insect control. FEBS Lett. , 540:81-85.

 

Regev, A., Rivkin, H., Inceoglu, B., Gershburg, E., Hammock, B. D., Gurevitz, M. and Chejanovsky, N. (2003) Further enhancement of baculovirus insecticidal efficacy with scorpion toxins that interact cooperatively. FEBS Lett . , 537:106-110.
Marcus, Y., Altman-Gueta, H., Finkler, A. and Gurevitz, M. (2003) The dual role of cysteine172 in redox regulation of Rubisco activity and degradation . J. Bacteriol . , 185:1509-1517.

 

Gordon, D. and Gurevitz, M. (2003) The selectivity of scorpion alpha-toxins for sodium channel subtypes is determined by subtle variations at the interacting surface. Toxicon , 41:125-128.

 

Gurevitz, M., Zilberberg, N., Froy, O., Turkov, M., Vilunsky, R., Karbat, I., Anglister, J., Shaanan, B., Pelhate, M., Adams, M. E., Gilles, N., and Gordon, D. (2002) Diversification of toxic sites on a conserved protein scaffold: a scorpion recipe for survival. In Perspectives in Molecular Toxinology (Menez, A., ed.), pp. 239-253, Wiley and Sons, Chichester, UK.

 

Gordon, D., Gilles, N., Bertrand, D., Molgo, J., Nicholson, G. N., Sauviat, M. P., Shichor, I., Lotan, I., Gurevitz, M., Kallen, R. G. and Heinemann, S. H. (2002) Scorpion toxins differentiating among neuronal sodium channel subtypes: Nature’s guide for design of selective drugs. In Perspectives in Molecular Toxinology (Menez, A., ed.), pp. 215-238, Wiley and Sons, Chichester, UK.

 

Gilles, N., Harrison, G., Karbat, I., Gurevitz, M., Nicholson, G. M. and Gordon, D. (2002) Variations in receptor site-3 on rat brain and insect sodium channels highlighted by binding of a funnel-web spider delta-Atracotoxin. Eur. J. Biochem. , 269:1500-1510.

 

Gurevitz, M., Gordon, D., Ben-Natan, S., Turkov, M. and Froy, O. (2001) Diversification of neurotoxins by C-tail ‘wiggling’ - a scorpion recipe for survival. FASEB J. , 15:1201-1205.

 

Gilles, N., Krimm, I., Bouet, F., Froy, O., Gurevitz, M., Lancelin, J-M. and Gordon, D. (2000) Structural implications on the interaction of scorpion alpha-like toxins with the sodium channel receptor site inferred from toxin iodination and pH-dependent binding. J. Neurochem. , 75:1735-1745.

 

Marcus, Y. and Gurevitz, M. (2000) Activation of cyanobacterial RuBP-carboxylase/ oxygenase is facilitated by inorganic phosphate via two distinct mechanisms. Eur. J.Biochem. , 267:5995-6003.

 

Lee, D., Gurevitz, M. and Adams, M. E. (2000) Modification of synaptic transmission and sodium channel inactivation by the insect-selective scorpion toxin Lqh-alpha-IT. J. Neurophysiol. , 83:1181-1187.

 

Froy, O., Zilberberg, N., Chejanovsky, N., Anglister, J., Loret, E., Shaanan, B., Gordon, D. and Gurevitz, M. (2000) Scorpion neurotoxins: Structure/function relationships and application in agriculture. Pest. Manag. Sci. , 56:472-474.

 

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