Prof. Yoav Gothilf

Department of Neurobiology
מחלקה לנוירוביולוגיה סגל אקדמי בכיר
Prof. Yoav Gothilf
Phone: 03-6406329
Another phone: 03-6409041
Fax: 03-6406329
Office: Sherman - Life Sciences, 404

Research Interests

Research topic: The circadian clock system: underlying molecular mechanisms, functional development and light entrainment. Hypothalamic control of sexual maturation and reproduction. Zebrafish as a model to study Alzheimer’s disease.


Research methods: Molecular genetics in whole animal, the zebrafsh;  Transgenesis and gene manipulation; Genome wide and bioinformatics analyses (in collaboration with experts); Behavior.


Main projects in the lab include:

  1. Mechanisms underlying circadian rhythms of gene expression
  2. Mechanisms underlying light-entrainment of the circadian clock
  3. Functions of novel genes in the zebrafish clock organ
  4. Development and plasticity of the kisspeptin and GnRH neuronal systems during development and sexual maturation
  5. Phenotype of transgenic zebrafish carrying Alzheimer’s disease-related human genes


Recent Publications

44. Ben-Moshe Z, Foulkes SN, Gothilf Y (2014). Functional development of the circadian clock in the zebrafish pineal gland. BioMed Research International; doi: 10.1155/2014/235781.


43. Ben-Moshe Z, Alon S, Mracek P, Faigenbloom L, Tovin A, Vatine G, Eisenberg E, Foulkes SN, Gothilf Y (2014). The light-induced transcriptome of the zebrafish pineal gland reveals complex regulation of the circadian clockwork by light. Nucleic Acids Research; doi: 10.1093/nar/gkt1359.


42. Mracek P, Santoriello C, Idda ML, Pagano C, Ben-Moshe Z, Gothilf Y, Vallone D, Foulkes NS (2012). Regulation of per and cry genes reveals a central role for the D-box enhancer in light-dependent gene expression. PLOS One 7(12):e51278.


41. Smadja-Storz S, Tovin A, Mracek P, Alon S, Foulkes NS, Gothilf Y (2012). Casein kinase 1delta activity: a key element in the zebrafish circadian timing system. PLOS ONE 8(1):e54189.


40. Dresner E, Zemel A, Arviv C, Barak SL, Alon S, Ofir R, Gothilf Y, Gozes I (2012). Novel Evolutionary-Conserved Role for the ADNP Protein Family that is Important for Erythropoiesis. J Biol Chem 287(48):40173-40185.


39. Tovin A, Alon S, Ben-Moshe Z, Mracek P, Vatine G, Foulkes N, Jacob-Hirsch J, Rechavi G, Toyama R, Coon SL, Klein DC, Eisenberg E, Gothilf Y (2012). Systematic identification of rhythmic genes reveals camk1gb as a new element in the circadian clockwork. PLOS Genetics 8(12):e1003116.


38. Idda ML, Bertolucci C, Vallone D, Gothilf Y, Sánchez-Vázquez FJ, Foulkes NS (2012).Circadian clocks: lessons from fish. Prog Brain Res. 199:41-57.


37. Strähle U, Bally-Cuif L, Kelsh R, Beis D, Mione M, Panula P, Figueras A, Gothilf Y, Brösamle C, Geisler R, Knedlitschek G (2012). EuFishBioMed (COST Action BM0804): A European Network to Promote the Use of Small Fishes in Biomedical Research. Zebrafish. 9 (2):90-3.


36. Stiebel-Kalish H, Reich E, Rainy N, Vatine G, Nisgav Y, Tovar A, Gothilf Y, Bach M (2012).Gucy2f zebrafish knockdown - a model for Gucy2d-related leber congenital amaurosis. Eur J Hum Genet. doi: 10.1038/ejhg.2012.10.


35. Zilberman-Peled B, Bransburg-Zabary S, Klein DC, Gothilf Y (2011). Molecular evolution of multiple arylalkylamine N-acetyltransferase (AANAT) in fish. Mar Drugs. 2011:906-21.


34. Vatine G, Vallone D, Gothilf Y, Foulkes NS (2011). It's time to swim! Zebrafish and the circadian clock. FEBS Lett. 585(10):1485-94.


33. Ben-Moshe Z, Vatine G, Alon S, Tovin A, Mracek P, Foulkes N and Gothilf Y (2010). Multiple PAR and E4BP4 bZIP transcription factors in zebrafish: diverse spatial and temporal expression patterns. Chronobiology Int. 27(8):1509-31.


32. Appelbaum L, Wang GX, Maro GS, Mori R, Tovin A, Marin W, Yokogawa T, Kawakami K, Smith SJ, Gothilf Y, Mignot E, Mourrain P (2009). Sleep-wake regulation and hypocretin-melatonin interaction in zebrafish. Proc. Natl. Acad. Sci. USA. 106(51):21942-7.


31. Abraham E, Palevitch O, Gothilf Y, Zohar Y (2010). Targeted gonadotropin-releasing hormone-3 neuron ablation in zebrafish: effects on neurogenesis, neuronal migration, and reproduction. Endocrinology. 151(1):332-40.


30. Vatine G, Vallone D, Appelbaum L, Mracek P, Ben-Moshe Z, Lahiri K, Gothilf Y and Foulkes NS (2009). Light directs zebrafish period2 expression via conserved D and E boxesPLoS Biology, 7(10): p. e1000223.


29. Alon S, Eisenberg E, Jacob-Hirsch J, Rechavi G, Vatine G, Toyama R, Coon SL, Klein DC, Gothilf Y (2009). A new cis-acting regulatory element driving gene expression in the zebrafish pineal gland . Bioinformatics, 25(5):559-62.


28. Palevitch O, Abraham E, Borodovsky N, Levkowitz G, Zohar Y and Gothilf Y (2009).Cxcl12a-Cxcr4b signaling is important for proper development of the forebrain GnRH system in zebrafish. Gen Comp Endocrinol. 165(2): p. 262-8.


27. Palevitch O, Abraham E, Borodovsky N, Levkowitz G, Zohar Y, Gothilf Y (2009). Nasal embryonic LHRH factor plays a role in the developmental migration and projection of gonadotropin-releasing hormone 3 neurons in zebrafish . Dev Dyn. 238(1):66-75.


26. Abraham E, Palevitch O, Gothilf Y and Zohar Y (2009). The zebrafish as a model system for forebrain GnRH neuronal development . Gen Comp Endocrinol. 164(2-3): p. 151-60.


25. Abraham E, Palevitch O, Ijiri S, Du SJ, Gothilf Y, Zohar Y (2008). Early development of forebrain gonadotrophin-releasing hormone (GnRH) neurones and the role of GnRH as an autocrine migration factor. J. Neuroendocrinol. 20(3):394-405.


24. Levy O, Appelbaum L, Leggat W, Gothlif Y, Hayward DC, Miller DJ, Hoegh-Guldberg O (2007). Light-responsive cryptochromes from a simple multicellular animal, the coralAcropora millepora. Science. 318:467-470.


23. Zilberman-Peled B, Appelbaum L, Vallone D, Foulkes NS, Anava S, Anzulovich A, Coon SL, Klein DC, Falcon J, Ron B, Gothilf Y (2007). Transcriptional regulation of arylalkylamine-N-acetyltransferase-2 gene in the pineal gland of the gilthead seabream . J. Neuroendocrinol. 19:46-53.


22. Ziv L, Tovin A, Strasser D, Gothilf Y (2007). Spectral sensitivity of melatonin suppression in the zebrafish pineal gland . Exp. Eye Res. 84:92-99.


21. Palevitch O, Kight K, Abraham E, S, Zohar Y, Gothilf Y (2007). Ontogeny of the GnRH systems in zebrafish brain: in situ hybridization and promoter-reporter expression analyses in intact animals . Cell. Tissue Res. 327:313-322.


20. Vuilleumier R, Besseau L, Boeuf G, Piparelli A, Gothilf Y, Gehring WG, Klein DC, Falcón J (2006). Starting the zebrafish pineal circadian clock with a single photic transition. Endocrinology. 147(5):2273-9.


19. Appelbaum L, Gothilf Y (2006). Mechanism of pineal-specific gene expression: The role of E-box and photoreceptor conserved elements. Mol. Cell. Endocrinol. 252:27-33.


18. Ziv L, Gothilf Y (2006). Circadian time-keeping during early stages of development . Proc. Natl. Acad. Sci. USA. 103:4146-4151.


17. Ziv L, Gothilf Y (2006). Period2 expression pattern and its role in the development of the pineal circadian clock in zebrafish. Chronobiology Int. 23:101-112.


16. Appelbaum L, Vallone D, Anzulovich A, Ziv L, Tom M, Foulkes N, Gothilf Y (2006).Zebrafish arylalkylamine-N-acetyltransferase genes - targets for circadian-clock regulation . J. Mol. Endocrinol. 36:337-347.


15. Zilberman-Peled B, Ron B, Gross A, Finberg JPM, Gothilf Y (2006). A possible new role for fish retinal serotonin-N-acetyltransferase-1 (AANAT1): Dopamine metabolism. Brain Res. 1073:220-228.


14. Neuman A, Gothilf Y, Haim A, Ben-Aharon G, Zisapel N (2005). Nocturnal patterns and up-regulated excretion of the melatonin metabolite 6-sulfatoxymelatonin in the diurnal rodent Psammomys obesus post-weaning under a short photoperiod. Comp Biochem Physiol A Mol Integr Physiol. 142(3):297-307.


13. Ziv L, Levkovitz S, Toyama R, Falcon J, Gothilf Y (2005). Functional Development of the Zebrafish Pineal Gland: Light-Induced Expression of Period2 is Required for Onset of the Circadian Clock. J Neuroendocrinol. 17:314-320.


12. Appelbaum L, Anzulovich A, Baler R, Gothilf Y (2005). Homeobox-clock protein interaction in zebrafish. A shared mechanism for pineal-specific and circadian gene expression. J Biol Chem. 25:11544-11551.


11. Peled B, Benhar I, Coon SL, Ron B, Gothilf Y (2004). Duality of serotonin-N-acetyltransferase in the gilthead seabream ( Sparus aurata): Molecular cloning and characterization of recombinant enzymes. Gen. Comp. Endocrinol. 138:139-147.


10. Appelbaum L, Toyama R, Dawid IB, Klein DC, Baler R, Gothilf Y (2004). Zebrafish serotonin-N-acetyltransferase-2 gene regulation: Pineal-restrictive downstream module (PRDM) contains a functional E-box and three photoreceptor conserved elements. Mol. Endocrinol. 18:1210-1221.


9. Wong TS, Gothilf Y, Zmora N, Kight KE, Meiri I, Elizur A, Zohar Y (2004). Developmental expression of three forms of Gonadotropin-releasing hormone and ontogeny of hypothalamus-pituitary-gonadal axis in gilthead seabream ( Sparus aurata). Biol. Reprod. 71:1026-1035


8. Falcon J, Gothilf Y, Coon SL, Boeuf G, Klein DC (2003). Genetic, temporal and developmental differences between melatonin rhythm generating systems in the fish pineal organ and retina. J Neuroendocrinol. 15:1-5.


7. Okuzawa K, Gen K, Bruysters M, Bogerd J, Gothilf Y, Zohar Y, Kagawa H (2003). Seasonal variation of the three native gonadotropin-releasing hormone messenger ribonucleic acids levels in the brain of female red seabream. Gen Comp Endocrinol. 130(3):324-32.


6. Gothilf Y, Toyama R, Coon SL, Du SJ, Dawid IB, Klein DC (2002). Pineal-specific expression of Green Fluorescent Protein under the control of the serotonin-N-acetyltransferase gene regulatory regions in transgenic zebrafish . Dev. Dynam. 225:241-249.


5. Meiri I, Gothilf Y, Zohar Y, Elizur A (2002). Physiological changes in the spawning gilthead seabream, Sparus aurata, succeeding the removal of males. Exp Zool. 292(6):555-64.


4. Gothilf Y, Coon SL, Toyama R, Chitnis A, Namboodiri MAA, Klein DC (1999). Zebrafish serotonin N-acetyltransferase-2: Marker for development of pineal photoreceptors and circadian clock function. Endocrinology 140:4895-4903.


3. Chow MM, Kight KE, Gothilf Y, Alok D, Stubblefield J, Zohar Y (1998). Multiple GnRHs present in a teleost species are encoded by separate genes: analysis of the sbGnRH and cGnRH-II genes from the striped bass, Morone saxatilis. J. Mol. Endocrinol. 21:277-289.


2. Gothilf Y, Meiri I, Elizur A, Zohar Y (1997). Preovulatory changes in the levels of three gonadotropin-releasing hormone-encoding messenger ribonucleic acids (mRNAs), gonadotropin beta-subunit mRNAs, plasma gonadotropin, and steroids in the female gilthead seabream, Sparus aurata. Biol. Reprod. 57:1145-1154.


1. Gothilf Y, MunozCueto JA, Sagrillo CA, Selmanoff M, Chen TT, Kah O, Elizur A, Zohar Y (1996). Three forms of gonadotropin-releasing hormone in a perciform fish ( Sparus aurata): Complementary deoxyribonucleic acid characterization and brain localization. Biol. Reprod. 55:636-645.


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