Next Generation Sequencing

Using Illumina's High-Throughput Sequencing platforms

Next generation sequencing (NGS) has been the ultimate technology for receiving high-throughput genomic data, replacing traditional sequencing methods such as, Sanger sequencing, microarrays, etc. NGS gives high-output and accurate results, with fast turnaround time, applicable to various biological studies. 

 

The Genomics Research Unit (GRU) has two benchtop sequencers utilizing Illumina's advanced sequencing technology, available for all TAU researchers: 

The MiSeq System

The NextSeq 500 System

 

Applications MiSeq NextSeq 500
Large Whole-Genome Sequencing (human, plant, animal) no yes
Small Whole-Genome Sequencing (microbe, virus) yes yes
Exome Sequencing no yes
Targeted Gene Sequencing (amplicon, gene panel) yes yes
Whole-Transcriptome Sequencing no yes
Gene Expression Profiling with mRNA-Seq no yes
Targeted Gene Expression Profiling yes no
Long-Range Amplicon Sequencing* yes no
miRNA & Small RNA Analysis yes yes
DNA-Protein Interaction Analysis yes yes
Methylation Sequencing no yes
16S Metagenomic Sequencing yes yes

 

Specifications MiSeq NextSeq 500
Output Range 0.3–15 Gb 20–120 Gb
Run Time 5–55 hr 11–29 hr
Reads per Run 1–50 million 130–800 million
Read Length 1 x 36 bp -2 × 300 bp 1 × 75 bp - 2 × 150 bp
Samples per Run 1–384  1-96

* Key applications are marked in bold

 

We provide assistance with all NGS steps, from experiment design to library preparation protocols and data analysis at the TAU Bioinformatics Unit.

 

 

In agreement with Danyel Biotech, all TAU researchers will receive 12.5% discount on all Illumina reagent kits.

 

 

For more information please contact us.

 

 

Tel Aviv University, P.O. Box 39040, Tel Aviv 6997801, Israel
UI/UX Basch_Interactive