ABI 3500xl Genetic analyzer
Our method of sequencing utilizes the BigDye Terminator Cycle Sequencing Kit from AB. Here each of the Dideoxy Terminators has a fluorescent dye attached to it. The reacted DNA fragments is separated and the fluorescence is detected. Signals are sent to a computer and collected by ABI's Data Collection Software, then interpreted by AB's Analysis software.
Since June 2012 the Unit runs the AB 3500xl Genetic Analyzer as its main sequencing apparatus.
The AB 3500 Genetic Analyzer is also used for Fragment Analysis.
Here labeled primers are used in PCR amplification to generate fluorescent labeled fragments. The labeled fragments are than being separated on the capillary sequencer, an internal size standard is used in each capillary, enabling a very accurate and reproducible size calling.
GeneScanT 600 LIZ® Size Standard is the custom size standard we use and the data is analyzed by AB GeneScan software.
ABI 3500xl Genetic analyzer
The 3500 Series is setting a new standard in capillary electrophoresis—integrating a number of platform improvements designed for the optical and thermal subsystems, and pioneering an innovative consumables system approach.
The 3500xL 24-capillary platform can run a wide variety of applications, including de novo sequencing and resequencing (mutational profiling), as well as microsatellite analysis, MLPA™, LOH, MLST, AFLP® and SNP validation or screening. The majority of applications can be run on a single polymer and capillary array.
This 24-capillary Genetic Analyzer is specifically configured with software for both fragment analysis and sequence typing applications.
