Biacore T200
*Currently unavailable
For enquiries, please contact - Dr. Karin Smorodinsky-Atias
The information provided in this section is taken from "Biacore™ T200 - Operating Instructions", "Biacore™ T200 - Software Handbook" and "Biacore™ sensor surface - Handbook" by Cytiva.
Surface Plasmon Resonance (SPR)
This method involves attaching one interacting partner to the surface of a sensor chip, and then passing a sample containing the second interaction partner over the surface.
Binding of molecules to the sensor surface generates a response which is proportional to the bound mass, and can be monitored in real time.
Application
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Interaction specificity
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Interaction kinetics and affinity
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Drug discovery
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Antibody characterization
Requirements
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Biacore tubes with penetrable caps (various sizes)
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A Biacore sensor chip with the required surface modification
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Means to attach on of the interacting partners to the chip surface
Advantages
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Real time measurements
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High sensitivity
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Quantitative method
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Relatively high throughput
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Sensor chips can be reused
