Cell Sorting

 

Flow cytometry
 

Flow Cytometry is the measurement of single cells as they flow one by one past laser beams  with the resulting light scatter and fluorescence emission detected and analyzed.  Multiple biophysical properties of each individual cell can be measured at rates of thousands  of cells per second. Cells are identified by their staining with fluorescent dyes that bind directly  to specific constituents like DNA, RNA, proteins or ions or with dyes conjugated to molecules  like antibodies, lectins, or hormones that bind to different cellular structures.  Cells may also be identified following an infection or tranfection by marker fluorescent proteins.  Flow Cytometry can detect and analyze multiple fluorescent staining simultaneously in each  individual cell and can report and physically separate them by these qualities.               

 

The Flow Cytometry unit has  FACSort with 2 lasers: 488nm and 635nm. 

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