Flow Cytometry is the measurement of single cells as they flow one by one past laser beams with the resulting light scatter and fluorescence emission detected and analyzed. Multiple biophysical properties of each individual cell can be measured at rates of thousands of cells per second. Cells are identified by their staining with fluorescent dyes that bind directly to specific constituents like DNA, RNA, proteins or ions or with dyes conjugated to molecules like antibodies, lectins, or hormones that bind to different cellular structures. Cells may also be identified following an infection or tranfection by marker fluorescent proteins. Flow Cytometry can detect and analyze multiple fluorescent staining simultaneously in each individual cell and can report and physically separate them by these qualities.
The Flow Cytometry unit has FACSort with 2 lasers: 488nm and 635nm.