Chirascan V100
Location - Britannia 107
For enquiries, please contact - Dr. Karin Smorodinsky-Atias
The information provided in this section is taken from “Chirascan V100 User Manual” by Applied Photophysics and "How to study proteins by circular dichroism". Kelly, S.M., T.J. Jess, and N.C. Price, Biochim Biophys Acta, 2005. 1751(2): p. 119-39.
Applied Photophysics Interactive widget for designing an experiment
Sample concentration
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In the far-UV region (<260 nm) - proteins have an extinction coefficient of about 7,000 M-1 cm-1. Therefore, the protein concentration should be approximately 0.2 to 0.4 mg/ml.
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In the near-UV region (>260 nm) - the absorbance of proteins depends mainly on the concentrations of the aromatic amino acids, particularly tryptophan. Therefore, the protein concentration should be approximately 0.45 mg/ml for proteins with a high tryptophan content, and ≥2.5 mg/ml for proteins with low tryptophan content.
Sample volume
The volume of the sample is determined by the size of the cuvette.
