Monolith NT.115

Location - Britannia 107

For enquiries, please contact -  Dr. Karin Smorodinsky-Atias

The information provided in this section is taken from “User Manual for the Monolith NT.115” and “User Starting Guide for the Monolith NT.115” by NanoTemper

 

Sample concentration, volume and dilutions

  • Prepare a 16 step dilution series around the expected Kd.

    • Start from a concentration of 20-fold the expected Kd, down to 0.02-fold the expected Kd.

  • The concentration of the fluorescently labeled molecule is kept constant and the concentration of the unlabeled binding partner is varied.

    • Try using the lowest concentration possible of the labeled molecule, to reduce the amount of unlabeled protein required to reach 20-fold expected Kd.

 

 

 

 

 

 

 

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