DNA Sequencing
No. |
Primer Name | Sequence | Size (mer) | Tm °C | Remarks |
|---|---|---|---|---|---|
1 |
M13/pUC | 5'AGCGGATAACAATTTCACACAGGA3' | 24 | 70 | reverse primer(-48) |
2 |
M13/pUC | 5'AACAGCTATGACCATGATTACG3' | 22 | 55 | reverse primer(-21) |
3 |
M13/pUC | 5'TGTAAAACGACGGCCAGT3' | 18 | 57 | uviversal primer(-20) |
4 |
M13/pUC | 5'GTTTTCCCAGTCACGACGTTG3' | 21 | 60 | universal primer(-40) |
5 |
T3 | 5'GCGCGAAATTAACCCTCACTAAAG3' | 24 | 69 | T3 |
6 |
T7 | 5'TTAATACGACTCACTATAGG3' | 20 | 62 | T7 |
7 |
SP6 | 5'CCAAGCTATTTAGGTGACAC3' | 20 | 55 | SP6 |
8 |
SP6A | 5'GCATTTAGGTGACACTATAG3' | 20 | 64 | for pcDNA3 ( reverse) |
9 |
BGH rev | 5'TAGAAGGCACAGTCGAGG3' | 18 | 55 | for pcDNA3 (reverse) |
10 |
pET | 5'TAGGGGAATTGTGAGCGGATAAC3' | 23 | 62 | for pET |
11 |
T7 Terminator | 5'GCTAGTTATTGCTCAGCGGT3' | 20 | 68 | for pET |
12 |
pGEX5 | 5'GGGCTGGCAAGCCACGTTTGGTG3' | 23 | 79 | 5' |
13 |
pGEX3 | 5'CCGGGAGCTGCATGTGTCAGAGG3' | 23 | 79 | 3' |
14 |
POLY-T | 5'TTTTTTTTTTTTTTTTTTTTTTV3' | 23 | 53 | for sequencing down stream poly-T regions |
15 |
POLY-A | 5'AAAAAAAAAAAAAAAAAAAAAB3' | 23 | 53 | for sequencing down stream poly-A regions |
16 |
pJET for | 5'CGACTCACTATAGGGAGAGCGGC3' | 23 | 77 | pJET forward |
17 |
pJET rev | 5'AAGAACATCGATTTTCCATGGCAG3' | 24 | 70 | pJET forward |
