Manufacturer: NanoTemper - Technologies
Location: Britannia 12
Internal User Price: 80NIS/hour
External User Price: 100$/run
Acquisition Date: 2015
Microscale Thermophoresis is a powerful method to quantify biomolecular interactions. It measures the motion of molecules along microscopic temperature gradients and detects changes in their hydration shell, charge or size. By combining the precision of fluorescence detection with the flexibility and sensitivity of thermophoresis, MST provides a flexible, robust and fast way to measure molecular interactions.
When performing a MST experiment, a microscopic temperature gradient is induced by an infrared laser, and the directed movement of molecules is detected and quantified using either covalently attached dyes, fluorescent fusion proteins or intrinsic tryptophan fluorescence.
The applications range from small-molecule binding events to protein-protein interactions and interactions of multi-protein complexes.