TAU Zebrafish Research Unit

Genetic manipulation 
 

Zebrafish one-cell embryo injection. 


 

Transgenic fish generation
 

GFP expression driven by actin promoter in 3 days post fertilization zebrafish larva. Scale indicates 500µm.

GFP expression driven by virus promoter in zebrafish early developmental stages. Scale indicates 200µm.

Genome editing by Crispr/Cas9

Characterization of knockout zebrafish. 

Expression in specific organs
 

 GFP expression driven by a liver promoter in 5-month-old zebrafish. Scale indicates 2mn.

GFP expression in sensory organs: olfactory system (red arrows) lateral line (yellow arrows) Scale indicates 200µm. 

Whole-mount In-Situ Hybridization

Liver enzyme transcripts in 4 days post fertilization larva. Scale indicates 200µm.

 

Pituitary hormone-encoding Lim-homeobox 4 (Lhx4) transcripts in 48 hours post fertilization embryos⁽¹⁾ Scale indicates 100µm.



Roisman-Geller, N.; Pisanty, O.;Weinberger, A.; Gajbhiye, D.S.;Golan, M.; Gothilf, Y. Combined Pituitary Hormone Deficiency in lhx4-Knockout Zebrafish. Int. J. Mol.Sci. 2024, 25, 7332.https://doi.org/10.3390/ijms25137332