TAU Zebrafish Research Unit

Genetic manipulation 
 

Zebrafish one-cell embryo injection. 


 

Transgenic fish generation
 

GFP expression driven by actin promoter in 3 days post fertilization zebrafish larva. Scale indicates 500µm.

GFP expression driven by virus promoter in zebrafish early developmental stages. Scale indicates 200µm.

Genome editing by Crispr/Cas9

Characterization of knockout zebrafish. 

Expression in specific organs
 

 GFP expression driven by a liver promoter in 5-month-old zebrafish. Scale indicates 2mn.

GFP expression in sensory organs: olfactory system (red arrows) lateral line (yellow arrows) Scale indicates 200µm. 

Whole-mount In-Situ Hybridization

Liver enzyme transcripts in 4 days post fertilization larva. Scale indicates 200µm.

 

Pituitary hormone-encoding Lim-homeobox 4 (Lhx4) transcripts in 48 hours post fertilization embryos⁽¹⁾ Scale indicates 100µm.

Rachel Zamostiano, Odelia Pisanty, Japhette Esther Kembou-Ringert, Reem Abu Rass, Avi Eldar, Marcelo Ehrlich, Yoav Gothilf , Eran Bacharach. 
The snakehead retrovirus promoter functions independently of the 3'ORF protein and its products are maternally inherited in transgenic zebrafish
PMID: 40504871 PMCID: PMC12193657 DOI: 10.1371/journal.ppat.1013243Affiliations Expand
Published: June 12, 2025, https://doi.org/10.1371/journal.ppat.1013243

Roisman-Geller, N.; Pisanty, O.;Weinberger, A.; Gajbhiye, D.S.;Golan, M.; Gothilf, Y.
Combined Pituitary Hormone Deficiency in lhx4-Knockout Zebrafish.
Int. J. Mol.Sci. 2024, 25, 7332. https://doi.org/10.3390/ijms25137332 


Rima Siauciuinate , Christelle Etard , Almut Koehler , Odelia Pisanty, Miriam Otto, Thomas Dickmeis , Olivier Kassel , Yoav Gothilf , Nicholas S Foulkes, Daniela Vallone
A simple and rapid shaking-based assay to genotype live, early developmental stage zebrafish embryos
https://doi.org/10.1177/00236772251351087