Prof. Eilon Shani
|2003-2005||B.Sc.||Hebrew University of Jerusalem||Robert H. Smith Faculty of Agriculture, Food and Environment|
|2006-2010||Ph.D.||Hebrew University of Jerusalem||Robert H. Smith Faculty of Agriculture, Food and Environment|
|2011-2013||Post-Doctoral fellow||Cell and Developmental Biology||University of California San Diego|
|2013-2018||Senior Lecturer||School of Plant Sciences and Food Security||Tel Aviv University|
|Since 2018||Prof.||School of Plant Sciences and Food Security||Tel Aviv University|
Other Appointments and Awards
|2015||Tel Aviv University - Teaching excellence appreciation|
|2016||The Marker Magazine, [40 under 40]|
|2017||Krill Prize – Wolf foundation|
|2017||Tel Aviv University - Rector award for excellence in teaching.|
|2018-present||Member of the Israeli Young Academy|
Plant Hormone Transport - Plant growth and development is mediated to a large extent by hormones. Plants regulate hormone response pathways at multiple levels including biosynthesis, metabolism, perception, and signaling. In addition, plants exhibit the unique ability to spatially regulate hormone distribution. This ability is illustrated most clearly in the case of auxin (IAA). The combined activity of auxin influx and efflux carrier proteins generates auxin maxima and local gradients that inform developmental patterning. The regulation of the cellular localization of PIN-FORMED (PIN) efflux transporters determines the direction of auxin flow from one cell to another. Until recently, little was known about the transport mechanisms and the distribution patterns of hormones other than auxin. These are exciting days for the plant hormone community as novel gibberellin (GA), abscisic acid (ABA), and cytokinin (CK) transporters are currently been identified, joining earlier findings on auxin transporters.
We are interested in the molecular factors regulating plant hormones transport mechanisms. We study the first events of cellular signaling - all the way to the patterning of the whole plant, primarily focusing on the plant hormones auxin, abscisic acid and gibberellin.
Redundancy of plant genomes - Plant genomes are highly redundant. For example, 80% of Arabidopsis genes (22,020 of the ~25,500 total genes) belong to families with at least two members. As a result, most single null mutants do not present an evident phenotype as the overlapping function of one or more paralogs mask any effects. During the past two decades, genetic variation and forward genetics screen have been expanded by creating random mutagenized lines using chemical or radiation treatments leading to the identification of novel genetic processes. However, these approaches cannot overcome the genetic redundancy problem a large fraction of the potential phenotypic plasticity is “hidden”.
We aim to Investigate how plants balance robustness and specialization in hormone transport at the cellular and subcellular levels. Our group places key technological challenges that require interdisciplinary expertise to overcome the long-standing obstacle of functional redundancy in plants. We use genome-scale artificial microRNAs (amiRNAs) and CRISPR technologies to generate mutants that allow us to identify the missing redundant hormone transporters to investigate the biological relevance of such specialized activity. In vitro transport assays and analyses of in vivo physiological responses reveal how gene families have developed complex robustness but diverse specialization.
Hu Y, Patra P, Pisanty O, Shafir A, Belew ZM, Binenbaum J, Ben Yaakov S, Shi B, Charrier L, Hyams G, Zhang Y, Trabolsky M, Caldararu O, Weiss D, Crocoll C, Avni A, Vernoux T, Geisler M, Nour-Eldin HH, Mayrose I, and Shani E (2023). Multi-Knock – a multi-targeted genome-scale CRISPR toolbox to overcome functional redundancy in plants. Nature Plants.
Binenbaum J, Wulff N, Camut L, Kiradjiev K, Anfang M, Tal I, Vasuki H, Zhang Y, Sakvarelidze-Achard L, Davière JM, Ripper D, Carrera E, Manasherova E, Ben Yaakov S, Lazary S, Hua C, Novak V, Crocoll C, Weinstain R, Cohen H, Ragni L, Aharoni A, Band LR, Achard P, Nour-Eldin HH and Shani E (2023). Gibberellin and abscisic acid transporters facilitate endodermal suberin formation in Arabidopsis. Nature Plants.
Zhang Y, Berman A, Shani E (2023). Plant Hormone Transport and Localization: – Signaling Molecules on the Move. Annual Review of Plant Biology.
Chen J, Hu Y, P Hao P, Zhang Y, Roth O, Njo M, Sterck L, Hu Y, Zhao Y, Geisler M, Shani E, Beeckman T, Vanneste S (2023). ABCB-mediated shootward auxin transport feeds into the root clock. EMBO Reports.
Mehra P, Pandey BK, Melebari D, Banda J, Leftley N, Couvreur V, Rowe J, Anfang M, De Gernier H, Morris E, Sturrock CJ, Mooney SJ, Swarup R, Faulkner C, Beeckman T, Bhalerao R, Shani E, Jones AM, Dodd A.C, Sharp RE, Sadanandom A, Draye X, Bennett MJ. (2022). Hydraulic flux–responsive hormone redistribution determines root branching. Science.
Zhang Y, Vasuki H, Liu J, Bar H, Lazary S, Egbaria A, Ripper D, Charrier D, Mussa Z, Wulff N, Nour-Eldin HH, Aharoni A, Ragni L, Strader L, Sade N, Weinstain R, Geisler M, Shani E (2021). ABA homeostasis and long-distance translocation is redundantly regulated by ABCG ABA importers. Science Advances.
Anfang M and Shani E. (2021). Transport mechanisms of plant hormones. Current Opinion in Plant Biology.
Hu Y, Omary M, Hu Y, Doron O, Hoermayer L, Chen Q, Megides O, Chekli O, Ding Z, Friml J, Zhao Y, Tsarfaty I, Shani E (2021). Cell kinetics of auxin transport and activity in Arabidopsis root growth and skewing. Nature Communications.
Ding T, Zhang F, Wang J, Wang F, Liu J, Xie C, Hu Y, Shani E, Kong X, Ding Z, Tian H (2021). Cell‐type action specificity of auxin on Arabidopsis root growth. The Plant Journal.
Kazachkova Y, Zemach I, Panda S, Bocobza S, Vainer A, Rogachev I, Dong Y, Ben-Dor S, Veres D, Kanstrup C, Konstanze Lambertz S, Crocoll C, Hu Y, Shani E, Michaeli S, Nour-Eldin HH, Zamir D, Aharoni A (2021). The GORKY glycoalkaloid transporter is indispensable for preventing tomato bitterness. Nature Plants.
Wexler S, Schayek H, Rajendar K, Tal I, Shani E, Meroz Y, Dobrovetsky R, Weinstain R (2019). Characterizing gibberellin flow in planta using photocaged gibberellins. Chemical Science.
Hauser F, de O Ceciliato P.H, Lin Y.C, Guo D, Gregerson J.D, Abbasi N, Youhanna D, Park J, Dubeaux G, Shani E, Poomchongkho N, J.I Schroeder. (2019), A seed resource for screening functionally redundant genes and isolation of new mutants impaired in CO2 and ABA responses. Journal of Experimental Botany. Journal of Experimental Botany.
Zhang Y, Naser V, Pisanty O, Omary M, Wulff N, Charrier L, Tal I, Hauser F, Hao P, Roth O, Fromm H, Schroeder J, Geisler M, Nour-Eldin H.H, and Shani E (2018). A transportome-scale amiRNA-based screen identifies redundant roles of Arabidopsis ABCB6 and ABCB20 in auxin transport. Nature Communication.
Binenbaum J, Weinstain R, and Shani E (2018). Gibberellin localization and transport in plants. Trends in Plant Science.
Roth O, Alvarez J.P, Levy M, Bowman J.L, Ori N and Shani E (2018). The KNOXI gene SHOOT MERISTEMLESS regulates flower organ identity in plants. Plant Cell.
Hyams G, Abadi S, Avni A, Halperin E, Shani E, and Mayrose I (2018). Optimal design for the genome-editing of multiple members of a gene family using the CRISPR system. Journal of Molecular Biology.
Kong X, Tian H, Yu Q, Zhang F, Wang R, Gao S, Xu W, Liu J, Shani E, Zhang L, Zhang X and Ding Z (2017). PHB3 maintains root stem cell niche identity through ROS responsive AP2/ERF transcription factors in Arabidopsis. Cell report.
Morozov D, Tal I, Pisanty O, Shani E and Cohen Y (2017). Microstructure and Microstructural Changes in Plant Tissues by Advanced Diffusion Magnetic Resonance Imaging Techniques. Journal of Experimental Botany.
Shani E, Salehin M, Zhang Y, Sanchez S.E, Doherty C, Wang R, Mangado C.C, Song L, Tal I, Pisanty O, Ecker J.R, Kay S.A, Pruneda-Paz J, Estelle M. (2017). Plant stress tolerance requires auxin-sensitive Aux/IAA transcriptional repressors. Current Biology.
Schayek H, Shani E and Weinstain R. (2017). Highlighting Gibberellins Accumulation Sites in Arabidopsis thaliana Root Using Fluorescently Labeled Gibberellins. Plant Hormones: Methods and Protocols.
Buskila Y, Sela N, Teper-Bamnolker P, Tal I, Shani E, Weinstain R, Gaba V, Tam Y, Lers A. and Eshel D. (2016). Stronger sink demand for metabolites supports dominance of the apical bud in etiolated growth. Journal of Experimental Botany.
Naser V and Shani E. (2016). Auxin response under osmotic stress. Plant Molecular Biology.
Tal I, Zhang Y,Egevang Jørgensen M, Pisanty O, Barbosa I.C.R, Zourelidou M, Regnault T, Crocoll C, Erik C.O, Weinstain R, Schwechheimer C, Halkier B.A, Nour-Eldin H.H, Estelle M and Shani E. (2016). The Arabidopsis NPF3 protein is a GA transporter. Nature Communication.
Katz E, Nisani S, Yadav B.S, Woldemariam M.G, Shai B, Obolski U, Ehrlich M, Shani E, Jander G and Chamovitz D.A. (2015). The glucosinolate breakdown product indole-3-carbinol acts as an auxin antagonist in roots of Arabidopsis thaliana. Plant Journal.
Matias-Hernandez L, Aguilar-Jaramillo A.E, Osnato M, Weinstain R, Shani E, Su P, and Pelaz S. (2016). TEMPRANILLO reveals the mesophyll as crucial for epidermal trichome formation. Plant Physiology.
Shani E, Weinstain R, Zhang Y, Castillejo C, Kaiserli E, Chory J, Tsien R.Y and Estelle M (2013). Gibberellins accumulate in the elongating endodermal cells of Arabidopsis root. PNAS.
Bargmann B.O.R, Vanneste S, Krouk G, Nawy T, Efroni I, Shani E, Choe G, Friml J, Estelle M, Birnbaum K.D (2013). A map of cell type-specific auxin responses. Molecular Systems Biology.
Sreeramulu S, Mostizky Y, Sunitha S, Shani E, Nahum H, Salomon D, Ben Hayun L, Gruetter C, Rauh D, Ori N, Sessa G (2013). BSKs are partially redundant positive regulators of brassinosteroid signaling in Arabidopsis. Plant Journal.
Ben-Gera H, Shwartz I, Shao MR, Shani E, Mark E and Ori N (2012). ENTIRE and GOBLET promote leaflet development in tomato by modulating auxin response. Plant Journal.
Burko Y, Geva Y, Refael-Cohen A, Shleizer-Burko S, Shani E, Berger Y, Halon E, Chuck G, Moshelion M and Ori N (2011). From organelle to organ: ZRIZI MATE-type transporter is an organelle transporter that enhances organ initiation. Plant Cell Physiol.
Fleishon S, Shani E, Ori N and Weiss D (2011). Negative reciprocal interactions between gibberellin and cytokinin in tomato. New Phytologist.
Yanai O, Shani E, Russ D and Ori N (2011). Gibberellin partly mediates LANCEOLATE activity in tomato. Plant Journal.
Shani E, Ben-Gera H, Shleizer-Burko S, Burko Y, Weiss D and Ori N (2010). Cytokinin regulates compound leaf development in tomato. Plant Cell.
Shani E, Burko Y, Ben-Yaacov L, Berger Y, Goldshmidt A, Sharon E and Ori N (2009). Stage-specific regulation of leaf maturation by KNOXI proteins. Plant Cell.
Shani E, Yanai O and Ori N (2006). The role of hormones in shoot apical meristem function. Current Opinion in Plant Biology.
Yanai O, Shani E, Dolezalb K, Tarkowskib P, Sablowskic R, Sandbergd G, Samacha A and Ori N (2005). Arabidopsis KNOXI proteins activate cytokinin biosynthesis. Current Biology.