Prof. Marcelo Ehrlich

מח חקר התא ואימונולוגיה סגל אקדמי בכיר
Prof. Marcelo Ehrlich
Phone: 03-6409406
Another phone: 03-6405174
Office: Britannia-Porter, 321

Research Interests

I am interested on the dynamic aspects of the cell's trafficking machinery, with a special focus on endocytic dynamics. More specifically, our study centers on the mechanisms by which the cell modulates vesicular traffic by altering:

  • The rate of formation of vesicular carriers, such as clathrin coated vesicles.
  • The spatio-temporal distribution of the process of vesicular carrier formation.
  • The make-up and structure of transport vesicles.

We will look at the modulatory effects of the following processes:

  • Viral infection. We are currently working with viruses from the Reoviridae family.
  • Cell attachment and spreading.
  • Epithelial to mesenchymal transition (EMT); a process essential for the metastatic progression of epithelial tumors.

In addition to the basic biochemical and cell biological techniques, we employ time-lapse microscopy with living cells as a mean to observe, characterize and quantify the cell's trafficking events.

Recent Publications

Liu X, Sun Y, Ehrlich M, Lu T, Kloog Y, Weinberg RA, Lodish HF, Henis YI: Disruption of TGF-beta growth inhibition by oncogenic ras is linked to p27Kip1 mislocalization. Oncogene 2000, 19(51):5926-5935.
 

Shenkman M, Ehrlich M, Lederkremer GZ: Masking of an endoplasmic reticulum retention signal by its presence in the two subunits of the asialoglycoprotein receptor. The Journal of Biological Chemistry 2000, 275(4):2845-2851.
 

Ehrlich M, Shmuely A, Henis YI: A single internalization signal from the di-leucine family is critical for constitutive endocytosis of the type II TGF-beta receptor. Journal of Cell Science 2001, 114(Pt 9):1777-1786.
 

Nohe A, Hassel S, Ehrlich M, Neubauer F, Sebald W, Henis YI, Knaus P: The mode of bone morphogenetic protein (BMP) receptor oligomerization determines different BMP-2 signaling pathways. The Journal of Biological Chemistry 2002, 277(7):5330-5338.
 

Yao D, Ehrlich M, Henis YI, Leof EB: Transforming growth factor-beta receptors interact with AP2 by direct binding to beta2 subunit. Molecular Biology of the Cell 2002, 13(11):4001-4012.
 

Chandran K, Parker JS, Ehrlich M, Kirchhausen T, Nibert ML: The delta region of outer-capsid protein micro 1 undergoes conformational change and release from reovirus particles during cell entry. Journal of Virology 2003, 77(24):13361-13375.
 

Hassel S, Schmitt S, Hartung A, Roth M, Nohe A, Petersen N, Ehrlich M, Henis YI, Sebald W, Knaus P: Initiation of Smad-dependent and Smad-independent signaling via distinct BMP-receptor complexes. The Journal of Bone and Joint Surgery 2003, 85-A Suppl 3:44-51.
 

Boll W, Ehrlich M, Collier RJ, Kirchhausen T: Effects of dynamin inactivation on pathways of anthrax toxin uptake. European Journal of Cell Biology 2004, 83(6):281-288.
 

Ehrlich M, Boll W, Van Oijen A, Hariharan R, Chandran K, Nibert ML, Kirchhausen T: Endocytosis by random initiation and stabilization of clathrin-coated pits. Cell 2004, 118(5):591-605.
 

Kfir S, Ehrlich M, Goldshmid A, Liu X, Kloog Y, Henis YI: Pathway- and expression level-dependent effects of oncogenic N-Ras: p27(Kip1) mislocalization by the Ral-GEF pathway and Erk-mediated interference with Smad signaling. Molecular and Cellular Biology 2005, 25(18):8239-8250.
 

Kirchhausen T, Boll W, van Oijen A, Ehrlich M: Single-molecule live-cell imaging of clathrin-based endocytosis. Biochemical Society Symposium 2005(72):71-76.
 

Nieland TJ, Ehrlich M, Krieger M, Kirchhausen T: Endocytosis is not required for the selective lipid uptake mediated by murine SR-BI. Biochimica et Biophysica Acta 2005, 1734(1):44-51.

From 2006 - (date of initiation of work at the Tel Aviv University – 10/2005)

 

Boucrot E, Saffarian S, Massol R, Kirchhausen T, Ehrlich M: Role of lipids and actin in the formation of clathrin-coated pits. Experimental Cell Research 2006, 312(20):4036-4048.
 

Eisenberg S, Shvartsman DE, Ehrlich M, Henis YI: Clustering of raft-associated proteins in the external membrane leaflet modulates internal leaflet H-ras diffusion and signaling. Molecular and Cellular Biology 2006, 26(19):7190-7200.
 

Macia E*, Ehrlich M*, Massol R, Boucrot E, Brunner C, Kirchhausen T: Dynasore, a cell-permeable inhibitor of dynamin. Developmental Cell 2006, 10(6):839-850.

16. Avezov E, Frenkel Z, Ehrlich M, Herscovics A, Lederkremer GZ: Endoplasmic Reticulum (ER) Mannosidase I Is Compartmentalized and Required for N-Glycan Trimming to Man5 6GlcNAc2 in Glycoprotein ER-associated Degradation. Molecular Biology of the Cell 2008, 19(1):216-225.
 

Chetrit D, Ziv N, Ehrlich M. Dab2 regulates clathrin assembly and cell spreading. The Biochemical Journal 2008, 1470-8728 (Electronic)
 

Eisenberg S, Giehl K, Henis YI, Ehrlich M: Differential interference of chlorpromazine with the membrane interactions of oncogenic K-Ras and its effects on cell growth. The Journal of Biological Chemistry 2008, 283(40):27279-88.
 

Rotblat B, Ehrlich M, Haklai R, Kloog Y: The Ras inhibitor farnesylthiosalicylic acid (Salirasib) disrupts the spatiotemporal localization of active Ras: a potential treatment for cancer. Methods in Enzymology 2008, 439:467-489.

 

Soria G, Yaal-Hahoshen N, Azenshtein E, Shina S, Leider-Trejo L, Ryvo L, Cohen-Hillel E, Shtabsky A, Ehrlich M, Meshel T et al: Concomitant expression of the chemokines RANTES and MCP-1 in human breast cancer: a basis for tumor-promoting interactions. Cytokine 2008, 44(1):191-200.
 

Danielli A, Arie A, Porat N, Ehrlich M: Detection of fluorescent-labeled probes at subpicomolar concentrations by magnetic modulation. Opt. Express 2008, 16, 19253-19259.
 

Rechtman MM, Nakaryakov A, Shapira KE, Ehrlich M, Henis YI: Rechtman MM, Nakaryakov A, Shapira KE, Ehrlich M, Henis YI. Different domains regulate homomeric and heteromeric complex formation among type I and type II transforming growth factor-beta receptors. The Journal of Biological Chemistry 2009; 284(12):7843-7852.
 

Horn G, Gaziel A, Wreschner DH, Smorodinsky NI, Ehrlich M: ERK and PI3K Regulate Different Aspects of the Epithelial to Mesenchymal Transition of Mammary Tumor Cells Induced by Truncated MUC1. Experimental Cell Research 2009; 315(8):1490-1504.
 

Mosesson Y, Chetrit D, Schley L, Berghoff J, Ziv T, Carvalho S, Milanezi F, Admon A, Schmitt F, Ehrlich M, Yarden Y. Monoubiquitinylation regulates endosomal localization of Lst2, a negative regulator of EGF receptor signaling. Developmental Cell 2009;16(5):687-698.
 

Degtyar E, Zusman T, Ehrlich M, Segal G. A Legionella effector acquired from protozoa is involved in sphingolipids metabolism and is targeted to the host cell mitochondria. Cellular Microbiology 2009.
 

Gottfried I, Ehrlich M, Ashery U. HIP1 exhibits an early recruitment and a late stage function in the maturation of coated pits. Cell Mol Life Sci 2009;66:2897-911.
 

Danielli A, Porat N, Arie A, Ehrlich M. Rapid homogenous detection of the Ibaraki virus NS3 cDNA at picomolar concentrations by magnetic modulation. Biosens Bioelectron. 2009 Dec 15;25(4):858-63. doi: 10.1016/j.bios.2009.08.047. Epub 2009 Sep 3. PubMed PMID: 19775882.

 

Danielli A, Porat N, Ehrlich M, Arie A. Magnetic modulation biosensing for rapid and homogeneous detection of biological targets at low concentrations. Curr Pharm Biotechnol. 2010 Jan;11(1):128-37. Review. PubMed PMID: 20214612.

 

Gottfried I, Ehrlich M, Ashery U. The Sla2p/HIP1/HIP1R family: similar structure, similar function in endocytosis? Biochem Soc Trans. 2010 Feb;38(Pt 1):187-91. doi: 10.1042/BST0380187. Review. PubMed PMID: 20074057.

 

Gottfried I, Ehrlich M, Ashery U. The Sla2p/HIP1/HIP1R family: similar structure, similar function in endocytosis? Biochem Soc Trans. 2010 Feb;38(Pt 1):187-91. doi: 10.1042/BST0380187. Review. PubMed PMID: 20074057.

 

Prizan-Ravid A, Elis E, Laham-Karam N, Selig S, Ehrlich M, Bacharach E. The Gag cleavage product, p12, is a functional constituent of the murine leukemia virus pre-integration complex. PLoS Pathog. 2010 Nov 11;6(11):e1001183. doi: 10.1371/journal.ppat.1001183. PubMed PMID: 21085616; PubMed Central PMCID: PMC2978732.

 

Chetrit D, Barzilay L, Horn G, Bielik T, Smorodinsky NI, Ehrlich M. Negative regulation of the endocytic adaptor disabled-2 (Dab2) in mitosis. J Biol Chem. 2011 Feb 18;286(7):5392-403. doi: 10.1074/jbc.M110.161851. Epub 2010 Nov 19. PubMed PMID: 21097498; PubMed Central PMCID: PMC3037652.

 

Sabo Y, Ehrlich M, Bacharach E. The conserved YAGL motif in human metapneumovirus is required for higher-order cellular assemblies of the matrix protein and for virion production. J Virol. 2011 Jul;85(13):6594-609. doi:10.1128/JVI.02694-10. Epub 2011 Apr 27. PubMed PMID: 21525358; PubMed Central PMCID: PMC3126496.

 

Barkan B, Kloog Y, Ehrlich M. Phenotypic reversion of invasive neurofibromin-deficient schwannoma by FTS: Ras inhibition reduces BMP4/Erk/Smad signaling. Mol Cancer Ther. 2011 Aug;10(8):1317-26. doi: 10.1158/1535-7163.MCT-10-1087. Epub 2011 Jun 1. PubMed PMID: 21632464.

 

Ivanovic T, Boulant S, Ehrlich M, Demidenko AA, Arnold MM, Kirchhausen T, Nibert ML. Recruitment of cellular clathrin to viral factories and disruption of clathrin-dependent trafficking. Traffic. 2011 Sep;12(9):1179-95. doi: 10.1111/j.1600-0854.2011.01233.x. Epub 2011 Jul 7. PMID: 21736684.

 

Ehrlich M, Horbelt D, Marom B, Knaus P, Henis YI. Homomeric and heteromeric complexes among TGF-? and BMP receptors and their roles in signaling. Cell Signal. 2011 Sep;23(9):1424-32. doi: 10.1016/j.cellsig.2011.04.004. Epub 2011 Apr14. Review. PubMed PMID: 21515362.

 

Barhoom S, Kaur J, Cooperman BS, Smorodinsky NI, Smilansky Z, Ehrlich M, Elroy-Stein O. Quantitative single cell monitoring of protein synthesis at subcellular resolution using fluorescently labeled tRNA. Nucleic Acids Res. 2011 Oct;39(19):e129. doi: 10.1093/nar/gkr601. Epub 2011 Jul 27. PubMed PMID: 21795382; PubMed Central PMCID: PMC3201886.

 

Eisenberg S, Beckett AJ, Prior IA, Dekker FJ, Hedberg C, Waldmann H, Ehrlich M, Henis YI. Raft protein clustering alters N-Ras membrane interactions and activation pattern. Mol Cell Biol. 2011 Oct;31(19):3938-52. doi: 10.1128/MCB.05570-11. Epub 2011 Aug 1. PubMed PMID: 21807892; PubMed Central PMCID: PMC3187366.

 

Berkovich R, Wolfenson H, Eisenberg S, Ehrlich M, Weiss M, Klafter J, Henis YI, Urbakh M. Accurate quantification of diffusion and binding kinetics of non-integral membrane proteins by FRAP. Traffic. 2011 Nov;12(11):1648-57. doi: 10.1111/j.1600-0854.2011.01264.x. Epub 2011 Aug 30. PubMed PMID: 21810156.

 

Sharfman M, Bar M, Ehrlich M, Schuster S, Melech-Bonfil S, Ezer R, Sessa G, Avni A. Endosomal signaling of the tomato leucine-rich repeat receptor-like protein LeEix2. Plant J. 2011 Nov;68(3):413-23. doi: 10.1111/j.1365-313X.2011.04696.x. Epub 2011 Aug 22. PubMed PMID: 21736652.

 

Benjamin S, Weidberg H, Rapaport D, Pekar O, Nudelman M, Segal D, Hirschberg K, Katzav S, Ehrlich M, Horowitz M. EHD2 mediates trafficking from the plasma membrane by modulating Rac1 activity. Biochem J. 2011 Nov 1;439(3):433-42. doi: 10.1042/BJ20111010. PubMed PMID: 21756249.

 

Viner R, Chetrit D, Ehrlich M, Segal G. Identification of two Legionella pneumophila effectors that manipulate host phospholipids biosynthesis. PLoS Pathog. 2012;8(11):e1002988. doi: 10.1371/journal.ppat.1002988. Epub 2012 Nov 1. PubMed PMID: 23133385; PubMed Central PMCID: PMC3486869.

 

Schmukler E, Shai B, Ehrlich M, Pinkas-Kramarski R. Neuregulin promotes incomplete autophagy of prostate cancer cells that is independent of mTOR pathway inhibition. PLoS One. 2012;7(5):e36828. doi: 10.1371/journal.pone.0036828. Epub 2012 May 14. PubMed PMID: 22606295; PubMed Central PMCID: PMC3351469.

 

Hirschhorn T, Barizilay L, Smorodinsky NI, Ehrlich M. Differential regulation of Smad3 and of the type II transforming growth factor-? receptor in mitosis: implications for signaling. PLoS One. 2012;7(8):e43459. doi: 10.1371/journal.pone.0043459. Epub 2012 Aug 22. PubMed PMID: 22927969; PubMed Central PMCID: PMC3425481.

 

Cabilly Y, Barbi M, Geva M, Marom L, Chetrit D, Ehrlich M, Elroy-Stein O. Poor cerebral inflammatory response in eIF2B knock-in mice: implications for the aetiology of vanishing white matter disease. PLoS One. 2012;7(10):e46715. doi: 10.1371/journal.pone.0046715. Epub 2012 Oct 4. PubMed PMID: 23056417; PubMed Central PMCID: PMC3464276.

 

Soria G, Lebel-Haziv Y, Ehrlich M, Meshel T, Suez A, Avezov E, Rozenberg P, Ben-Baruch A. Mechanisms regulating the secretion of the promalignancy chemokine CCL5 by breast tumor cells: CCL5's 40s loop and intracellular glycosaminoglycans. Neoplasia. 2012 Jan;14(1):1-19. PubMed PMID: 22355269; PubMed Central PMCID: PMC3281937.

 

Nachmias D, Sklan EH, Ehrlich M, Bacharach E. Human immunodeficiency virus type 1 envelope proteins traffic toward virion assembly sites via a TBC1D20/Rab1-regulated pathway. Retrovirology. 2012 Jan 19;9:7. doi: 10.1186/1742-4690-9-7. PubMed PMID: 22260459; PubMed Central PMCID: PMC3283470.

 

Moskovich O, Herzog LO, Ehrlich M, Fishelson Z. Caveolin-1 and dynamin-2 are essential for removal of the complement C5b-9 complex via endocytosis. J Biol Chem. 2012 Jun 8;287(24):19904-15. doi: 10.1074/jbc.M111.333039. Epub 2012 Apr 23. PubMed PMID: 22528500; PubMed Central PMCID: PMC3370175.

 

Ehrlich M, Gutman O, Knaus P, Henis YI. Oligomeric interactions of TGF-? and BMP receptors. FEBS Lett. 2012 Jul 4;586(14):1885-96. doi: 10.1016/j.febslet.2012.01.040. Epub 2012 Jan 27. Review. PubMed PMID: 22293501.

 

Shapira KE, Gross A, Ehrlich M, Henis YI. Coated pit-mediated endocytosis of the type I transforming growth factor-? (TGF-?) receptor depends on a di-leucine family signal and is not required for signaling. J Biol Chem. 2012 Aug 3;287(32):26876-89. doi: 10.1074/jbc.M112.362848. Epub 2012 Jun 15. PubMed PMID: 22707720; PubMed Central PMCID: PMC3411024.

 

Vernitsky H, Rechavi O, Rainy N, Besser MJ, Nagar M, Schachter J, Lerenthal Y, Ehrlich M, Kloog Y, Goldstein I. Ras oncoproteins transfer from melanoma cells to T cells and modulate their effector functions. J Immunol. 2012 Nov 1;189(9):4361-70. doi: 10.4049/jimmunol.1200019. Epub 2012 Oct 1. PubMed PMID: 23028055.

 

Elis E, Ehrlich M, Prizan-Ravid A, Laham-Karam N, Bacharach E. p12 tethers the murine leukemia virus pre-integration complex to mitotic chromosomes. PLoS Pathog. 2012 Dec;8(12):e1003103. doi: 10.1371/journal.ppat.1003103. Epub 2012 Dec 27. PubMed PMID: 23300449; PubMed Central PMCID: PMC3531515.

 

Tazat K, Harsat M, Goldshmid-Shagal A, Ehrlich M, Henis YI. Dual Effects of Ral-Activated Pathways on p27 Localization and TGF-? Signaling. Mol Biol Cell. 2013 Apr 10. [Epub ahead of print] PubMed PMID: 23576547.

 

 

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