Prof. Menachem Gutman

ביוכימיה וביולוגיה מולקו אמריטוס
Prof. Menachem Gutman
Phone: 03-6409875
Another phone: 03-6409824
Fax: 03-6409875
Office: Sherman - Life Sciences, 513

Research Interests

At the molecular level, all biological interactions are controlled by atoms located on the surface of macromolecular structures. The contacts between protein molecules and their surrounding water, determine the specificity and intensity of the interactions. Our scientific interest is at this atomistic level, looking at reactions that take place within the picosecond to nanosecond time frame over distances measured in Ǻ units.

The experimental system used in the Laser Laboratory for Fast Reactions (active since 1979) employed photo-excitation of photo acid molecules driving a sub-nanosecond acidification of the solution. The observations consisted of fast (microsecond) and ultra-fast (picoseconds) fluorescence and absorbance transitions, which were analyzed through integration of differential rate equations. These measurements were applied for probing defined spaces like the active site of proteins, the inter-membrane space in multi-lamellar bodies, the inner aqueous body of a reversed micelles and the solvation layer of protein.   Since 2005, the main line of the research is detailed understanding of molecular events at atomic resolution using Molecular Dynamics techniques coupled with experimental measurement. These lines of research are exemplified by the following research topics and the resulting publications.

 

1) Properties of water molecules at the interface of proteins and membranes, with an emphasis on MRI imaging of lipids in water. Collaboration with Dr. Aviv Mezer, Safra Center for Brain Sciences. Hebrew University, Jerusalem.

2) Mechanism of ion passage through channelrhodopsin. Collaboration with Prof. E. Bamberg. MPI for Biophysics Frankfurt, Germany.

3) Interaction of ions with proteins, with emphasize on the difference between the crystal s and solution structures. Collaboration with Dr. Yossi Tsfadia Life sciences Tel Aviv University.

Recent Publications

Amram S, Ganoth A, Tichon O, Peer D, Nachliel E, et al. (2014) Structural Characterization of the Drug Translocation Path of MRP1/ABCC1. Israel Journal of Chemistry 54: 1382-1393.

 

Levin L, Zelzion E, Nachliel E, Gutman M, Tsfadia Y, et al. (2013) A single disulfide bond disruption in the beta3 integrin subunit promotes thiol/disulfide exchange, a molecular dynamics study. PLoS One 8: e59175.

 

Azoulay I, Kucherenko N, Nachliel E, Gutman M, Azem A, et al. (2013) Tracking the Interplay between Bound Peptide and the Lid Domain of DnaK, Using Molecular Dynamics. Int J Mol Sci 14: 12675-12695.

 

Abramovitz A, Gutman M, Nachliel E (2012) Structural coupling between the Rho-insert domain of Cdc42 and the geranylgeranyl binding site of RhoGDI. Biochemistry 51: 715-723.

 

Radoszkowicz L, Presiado I, Erez Y, Nachliel E, Huppert D, et al. (2011) Time-resolved emission of flavin adenine dinucleotide in water and water-methanol mixtures. Phys Chem Chem Phys 13: 12058-12066.

 

 Agmon N, Gutman M (2011) Bioenergetics: Proton fronts on membranes. Nature Chem 3: 840-842.    

 

 

Tel Aviv University, P.O. Box 39040, Tel Aviv 6997801, Israel
UI/UX Basch_Interactive